Figure 4.

GSH antagonizes the generation of ROS in the supernatants of hepatocytes. (A) BRL-3A cells were treated with different levels of oxygen (5% O₂, 10% O₂ and 21% O₂) for 12 h. ROS in the supernatants was quantified using a colorimetric assay. Data were expressed as means ± SEM from 3 independent experiments. * p < 0.05, ** p < 0.01 vs. Control; (B) BRL-3A cells were treated with GSH (0, 0.5, 2.5, 10 mmol/L) and then exposed to 5% oxygen for 12 h. The ROS level in the hypoxia conditioned medium was analyzed. Data were expressed as means ± SEM from 3 independent experiments. * p < 0.05, *** p < 0.001 vs. group treated with 0 mmol/L GSH.