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. Author manuscript; available in PMC: 2011 Jun 30.
Published in final edited form as: J Immunol. 2010 Dec 29;186(3):1384–1390. doi: 10.4049/jimmunol.1002545

Figure 6.

Figure 6

Increased cell apoptosis in D− iNKT cell precursors. (A) Annexin V staining of iNKT cells in the thymus from D+ and D− mice. TCRβ and αGalCer-CD1d tetramer positive cells were gated. Data shown is one representative of 8 mice per group. Values are mean ± SEM. The percentage of annexin V+ iNKT cells is significantly different between D+WT and D−WT or D− CypKO mice (p<0.05). (B) Gating strategy for identifying DPdull iNKT cell precursors. The DPdull cells in the top panel are gated and evaluated for tetramer+ iNKT cells (bottom panel). The DPdull/tetramer+ cells are then gated. (C) CD24 and annexinV staining of iNKT cell precursors gated in B. Negative control staining for annexinV is shown in S1. Data represent eight mice per group. The percentage of CD24+DPdulltetramer+ cells is significantly different between D+WT and D− WT mice (p<0.01). The apoptosis is significantly higher in the D− iNKT cell precursors (CD24−DPdulltetramer+ cells, p<0.001).