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. 2011 Jul 6;101(1):237–244. doi: 10.1016/j.bpj.2011.04.054

Figure 2.

Figure 2

(a) Log plot of the amplification curves from the qPCR reaction. The threshold cycle (Ct) is the cycle number at which the fluorescent signal of the reaction crosses the threshold value. (b) The Ct value is inversely related to the amount of starting template. A plot of the Ct values versus time yields the growth curve for the target locus.