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. 2011 Jul 6;101(1):205–216. doi: 10.1016/j.bpj.2011.05.022

Figure 3.

Figure 3

Tubulin assembly in the presence of Spm leads to formation of short bundles. (A) Tubulin assembly assessed by turbidity (λ = 370 nm) for various Spm concentrations (25 μM tubulin). We remark that both the lag time to start polymerization and the time required to reach the plateau shorten as Spm concentration increases. (B) AFM images of MTs assembled for 30 min at 37°C in the presence of varying concentrations of Spm (25 μM tubulin). MT bundles are clearly detected at 300 μM Spm and at higher concentrations.