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. 2011 Jul 6;101(1):90–99. doi: 10.1016/j.bpj.2011.02.062

Figure 3.

Figure 3

(a) Monitoring the area of a single Lβ-phase domain in a single supported bilayer of a 1:1 DMPC/DSPC mixture visualized at 30°C during sPLA2 hydrolysis. (b) Burst behavior of the hydrolytic event occurring ∼60 min after addition of sPLA2, as monitored by measuring the area increase of the Lβ-phase domain. (c) Zoom-out after the hydrolytic burst to show the formation of holes in the supported bilayer due to loss of membrane components during the hydrolytic burst. Monitoring for 30 min after the hydrolytic burst shows that this structure is stable in the continued presence of sPLA2. The 40-Å height difference shown in the height profile (white line on image and lower graph) demonstrates that the dark areas represent holes in the membrane.