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Primer extension assay for TdT activity in extracts. A 32P-labeled 20mer (open arrow) was incubated with extract from CHOK1 (lanes 1–4) or xrs-6 (lanes 5–8) cells either transfected (+TdT) or not transfected (–TdT) with TdT expression vector. Reactions were stopped at the time points indicated. Arrows at the right indicate predominant TdT-dependent extension products. All lanes are from the same gel.
