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. 2011 May 1;7(5):477–489. doi: 10.4161/auto.7.5.14811

Figure 2.

Figure 2

Figure 2

A close-up view of the autophagy and lysosomal genes containing cis-acting elements of the E-box transcription factors SREBP1 and USF, AP-1 and NFE2. In the diagram, the symbol “V$” is placed before the IDs of transcription factor binding sites to distinguish them from the autophagy-lysosomal genes. Validation of regulation of autophagy-lysosomal genes by SREBP1. HEK293 cells were transfected by SREBP1 or TFEB cDNA. RNA was harvested by Trizol reagent at 24 hours post-transfection and passed quality control. Quantitative RT-PCR was performed in triplicate. SREBP1 and TFEB mRNA level were increased by 21- and 343-fold, respectively, by real-time RT-PCR assay. The mRNA levels of target genes were plotted in the bar graph. LAMP1, GABARAP, CLN3, CTSS , CTSD, CTNS, ATP6VOC, VPS 18 and PPT1 are upregulated by SREBP1. LAMP1, CLN3, ATP6VOC and PPT1 are upregulated by TFEB. *p < 0.05 by Student t-test compared to control empty vector transfection.