Figure 4.

Dual fluorescent protein, ROSELLA^Cyt, was restricted to the cytosol in Zn²⁺-treated cells. PS cells transformed with a plasmid encoding cytoplasmic ROSELLA²⁹ were grown to mid-exponential phase and treated for 16 h with 1.0 mM PMSF together with 13 mM ZnSO₄ or 0.22 µM rapamycin (Rap) as indicated. Only rapamycin, and rapamycin with zinc-treatments brought ROSELLA into vacuoles causing them to fluoresce red.