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Atg17 recruits Atg35 at the PNS during micropexophagy. The WT strains overexpressing eCFP-Atg17 (STN373), Atg35-eYFP (STN343) or both eCFP-Atg17 and Atg35-eYFP (STN346), and expressing the endogenous levels of Sec61-mCherry were grown overnight in SM (three top panels) and transferred to SD (three bottom panels) for 1 h. The peripheral ER and nuclear envelope were labeled with Sec61-mCherry and the PAS with eCFP-Atg17. Bar, 5 µm.
