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Atg28 mediates the interaction of Atg35 with Atg17. (A) Co-immunoprecipitation of Atg17, Atg28 and Atg35. The WT strain overexpressing Atg28-mCherry, Atg35-eYFP and HA-Atg17 (STN401) and the atg28Δ mutant overexpressing Atg35-eYFP and HA-Atg17 (STN387) were grown for 16 h in SM and transferred for 1 h to SD. Immunoprecipitation was performed as described in Materials and Methods. (B) Schematic view of Atg17, Atg28 and Atg35 interactions.
