Table 1.
P. pastoris strains and plasmids used in this study
| Name | Description | Genotype and plasmid | Source |
| GS115 | WT | his4 | 38 |
| GS200 | WT | arg4 his4 | 39 |
| PPS2 | atg28Δ | GS200 atg28Δ::ScARG4 | 13 |
| PPY12h | WT | arg4 his4 | 40 |
| R12 | atg1Δ | GS115 atg1-1::ZeocinR | 41 |
| SJCF1376 | WT | STN274 his4::pJCF419 (PATG8-GFP-ATG8, HIS4, HygromycinR) | This study |
| SJCF1472 | WT | PPY12h his4::pJCF549 (PGAPDH-ATG35, HIS4) | This study |
| SJCF1473 | WT | SJCF1472 HIS4::pJCF419 (PATG8-GFP-ATG8, HygromycinR) | This study |
| SJCF1474 | WT | PPY12h his4::pJCF552 (PYPT1-ATG35-eYFP, HIS4) | This study |
| SJCF1475 | WT | STN350 his4::pJCF552 (PYPT1-ATG35-eYFP, HIS4) | This study |
| SMD542 | WT | GS200 PAOX-β-GAL, ARG4 | 13 |
| SMD1163 | pep4 prb1 | pep4 prb1 his4 | 32 |
| STN66 | atg1Δ | R12 his4::pJCF208 (PATG8-GFP-ATG8, HIS4) | 12 |
| STN70 | WT | GS115 his4::pJCF208 (PATG8-GFP-ATG8, HIS4) | 12 |
| STN71 | atg1Δ | STN66 PAOX1::pRDM054 (PAOX1-BFP-SKL, HygromycinR) | This study |
| STN77 | atg28Δ | PPS2 his4::pJCF208 (PATG8-GFP-ATG8, HIS4) | 12 |
| STN78 | WT | STN70 PAOX1::pRDM054 (PAOX1-BFP-SKL, HygromycinR) | This study |
| STN87 | atg28Δ | STN77 PAOX1::pRDM054 (PAOX1-BFP-SKL, HygromycinR) | This study |
| STN120 | WT | PPY12h ATG28::pTYN5 (ATG28-GFP, HIS4) | This study |
| STN179 | atg35Δ | SVN3 PAOX1::pRDM054 (PAOX1-BFP-SKL, HygromycinR) | This study |
| STN227 | WT | PPY12h ATG35::pTYN14 (ATG35-eCFP, G418R) | This study |
| STN274 | WT | PPY12h his4::pTYN17 (PGAPDH-ATG35-eYFP, HIS4) | This study |
| STN315 | atg28Δ | PPS2 his4::pTYN17 (PGAPDH-ATG35-eYFP, HIS4) | This study |
| STN322 | WT | STN274 arg4::pJCF170 (PGAPDH-eCFP-ATG17, ARG4) | This study |
| STN338 | WT | STN274 arg4::pJCF477 (PATG8-mCHERRY-ATG8, ARG4) | This study |
| STN343 | WT | STN274 SEC61::pKSN256 (SEC61-mCHERRY, HygromycinR) | This study |
| STN346 | WT | STN322 SEC61::pKSN256 (SEC61-mCHERRY, HygromycinR) | This study |
| STN350 | WT | PPY12h arg4::pJCF170 (PGAPDH-eCFP-ATG17, ARG4) | This study |
| STN373 | WT | STN350 SEC61::pKSN256 (SEC61-mCHERRY, HygromycinR) | This study |
| STN385 | WT | STN274 his4::pTYN26 (PGAPDH-HA-ATG17, HIS4, HygromycinR) | This study |
| STN387 | atg28Δ | STN315 his4::pTYN26 (PGAPDH-HA-ATG17, HIS4, HygromycinR) | This study |
| STN401 | WT | STN385 arg4::pJCF500 (PGAPDH-ATG28-mCHERRY, ARG4) | This study |
| SVN1 | atg35Δ | GS200 atg35Δ::ScARG4 | This study |
| SVN2 | atg35Δ | SVN1 his4::pTYN10 (PATG35-ATG35, HIS4) | This study |
| SVN3 | atg35Δ | SVN1 his4::pJCF208 (PATG8-GFP-ATG8, HIS4) | This study |
| Y11430 | WT | Prototroph | Collection strain |