Figure 4.

Loss of both Bax and Bak facilitates oncogenic transformation. (A) bax^−/−bak^−/− MEF are resistant to death induced by loss of attachment and serum deprivation. MEF of different genotypes were cultured in uncoated bacterial culture dishes or in the absence of FBS for 48 h. The percentage of cell death was determined by PI-exclusion. (B–D) MEF of different genotypes were infected with viral constructs expressing adenovirus-5 E1A and an activated ras oncogene, and cultured in soft agar. Two weeks after infection, plates were photographed and the number of foci scored. (B) Photographs of representative plates. (C) Photographs of foci. (D) Graph quantifying the number of foci from three experiments.