Table 2.
Bacterial strains and plasmids
| Strain or plasmid
|
Relevant genotypea
|
Source, construction or referenceb
|
|---|---|---|
| B. subtilis strains | ||
| SG38 | spo+ | Errington and Mandelstam (1986) |
| 168 | spo+ | C. Anagnostopoulos |
| 700 | Ω(spoIIAB′-′lacZ cat) | Partridge et al. (1991) |
| 713 | spoIVA-lacZ cat | Stevens et al. (1992) |
| 1271 | Ω(yoaVW::spoIIQ-lacZ tet) | L.-J. Wu, unpub. |
| 1305 | spoIIE::pSG1902 (spoIIE-gfp cat) | Wu et al. (1998) |
| 1311 | minD::ermC spoIIE::pSG1902 (spoIIE-gfp cat) | pSG1902 → 1901 (Cm) |
| 1312 | minD::ermC divIVA::tet spoIIE::pSG1902 (spoIIE-gfp cat) | pSG1902 → 1920 (Cm) |
| 1756 | divIVA::pSG1043 (PdivIVA-lacZ ermC Pspac− divIVA) | Edwards and Errington (1997) |
| 1803 | divIVA::pSG1612 (PdivIVA-gfp divIVA+ cat) | pSG1612 → 168 (Cm) |
| 1806 | (φ105J19) spoIIAA-lacZ cat | φ105J19 → SG38 (Cm) |
| 1807 | minD::ermC (φ105J19) spoIIAA-lacZ cat | φ105J19 → 1901 (Cm) |
| 1808 | minD::ermC divIVA::tet (φ105J19) spoIIAA-lacZ cat | φ105J19 → 1920 (Cm) |
| 1809 | Ω(spoIIAB′-′lacZ cat) | 700 → 168 (Cm) |
| 1810 | gpr::pPS1326 (gpr-lacZ cat) | pPS1326 → SG38 (Cm) |
| 1813 | minD::ermC gpr::pPS1326 (gpr-lacZ cat) | pPS1326 → 1901 (Cm) |
| 1811 | minD::ermC divIVA::tet gpr::pPS1326 (gpr-lacZ cat) | pPS1326 → 1920 (Cm) |
| 1855 | spoIVA-lacZ cat | 713 → SG38 (Cm) |
| 1856 | minD::ermC spoIVA-lacZ cat | 713 → 1901 (Cm) |
| 1857 | minD::ermC divIVA::tet spoIVA-lacZ cat | 713 → 1920 (Cm) |
| 1865 | ΔdivIVA::cat minD::ermC Ω(amyE::Pxyl-gfp-minD spc) | M. Dyson and H.B.T., unpubl. |
| 1901 | minD::ermC | Edwards and Errington (1997) |
| 1920 | minD::ermC divIVA::tet | Marston and Errington (1999) |
| 3011 | ΔdivIVA::cat minD::ermC Ω(amyE::Pxyl-gfp-minD spc) Ω(yoaVW::spoIIQ-lacZ tet) | 1271 → 1865 (Tc) |
| 3012 | As 3011, chr::pSG1850 (divIVA+ kan) | pSG1850 → 1920 (Km) |
| 3013 | As 3011, chr::pSG1850 (divIVA13 kan) (N99D) | pSG1850* → 1920 (Km) |
| 3014 | As 3011, chr::pSG1850 (divIVA14 kan) (V25I) | pSG1850* → 1920 (Km) |
| 3015 | As 3011, chr::pSG1850 (divIVA15 kan) (promoter mutation) | pSG1850* → 1920 (Km) |
| 3016 | As 3011, chr::pSG1850 (divIVA16 kan) (L140F) | pSG1850* → 1920 (Km) |
| 3017 | ΔdivIVA::catΩ(yoaVW::spoIIQ-lacZ tet) chr::pSG1850 (divIVA13 kan) (N99D) | 3013 → 1271 (Km and Cm) |
| 3018 | As 3011, chr::pSG1850 (divIVA18 kan) (I136T) | pSG1850* → 1920 (Km) |
| Plasmids | ||
| pBEST502 | kan. Integration plasmid | |
| pPS1326 | gpr-lacZ cat. Integration plasmid | Sun et al. (1991) |
| pSG1612 | PdivIVA-gfp divIVA+ cat. Integration plasmid | Edwards et al. (2000) |
| pSG1850 | ylmH+ divIVA+ kan. Integration plasmid | See Material and Methods |
| pSG1902 | spoIIE-gfp cat. Integration plasmid | Wu et al. (1998) |
a
All strains carry the trpC2 marker.
b
For DNA transformations, the source of the DNA is shown, followed by an arrow, then the recipient strain, with the antibiotic selection in parentheses. Cm, chloramphenicol; Km, kanamycin; Tc, tetracycline.