Table 1.
Cell size, division and growth characteristics of wild type and mat3-4
| Strain
|
Daughter cell
size, μm3 (±S.D.)
|
Daughter cell protein
content, pg/cell
(±S.D.)
|
Average division number
|
Minimum commitment
size, μm3 (±S.D.)
|
Mass doubling time
(h)
|
|---|---|---|---|---|---|
| wild type | 113 ± 37 | 22 ± 2 | 1.6 | 178 ± 10 | 6.5 |
| mat3-4 | 23 ± 10 | 5.1 ± 0.3 | 2.6 | 110 ± 12 | 6.8 |
| mat3-4 + pMat3 | 115 ± 41 | 21 ± 2 | 1.6 | N.D. | N.D. |
Data for wild-type, mat3-4, and complemented mat3-4(+pMat3) strains. Daughter cell size and protein content was determined for gametes. Average division number was determined for cells grown exponentially in continuous light and then shifted to the dark as in Figure 3. Minimum commitment size was determined as described in Materials and Methods. Doubling time is for cells growing exponentially in continuous light.