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. Author manuscript; available in PMC: 2011 Jun 30.
Published in final edited form as: Angew Chem Int Ed Engl. 2009;48(27):4990–4992. doi: 10.1002/anie.200900858

Figure 1.

Figure 1

Intramololecular, time-resolved, fluorescence resonance energy transfer (TR-FRET) between eDHFR-bound TMP-TCs and GFP. Increasing concentrations of purified eDHFR-GFP were titrated against a constant concentration (20 nM) of each compound. Sensitized GFP emission (520 nm) was detected after a time delay of 100 μs, upon pulsed excitation with near-UV light (ca. 340 nm): green diamonds, TMP-Lumi4; red circles, TMP-cTTHA; blue x’s, TMP-cDTPA. Addition of 1 μM TMP reduced the signal, confirming FRET (shown for TMP-cTTHA, black squares). Lines represent non-linear least squares fit to the data.