Table 1. T4 Dam, T2 Dam and T2 Dam P126S steady-state kinetic parameters determined with synthetic oligonucleotides.
| Structure of | T4 Dam | T2 Dam | T2 Dam P126S | ||||||
| recognition site |
Km (nM) |
kcat (s–1 10–3) |
Relative kcat (%) |
Km (nM) |
kcat (s–1 10–3) |
Relative kcat (%) |
Km (nM) |
kcat (s–1 10–3) |
Relative kcat (%) |
| GATC | 10.3 (0.6) | 24.80 (4.6) | 100 | 13.9 (1.5) | 41.90 (4.3) | 169 | 11.0 (1.6) | 39.30 (3.5) | 158 |
| GATT | 4.4 (1.8) | 5.70 (0.4) | 23 | 8.6 (2.4) | 9.10 (1.2) | 37 | 16.3 (2.6) | 13.90 (1.1) | 56 |
| GATA | 5.7 (0.9) | 5.80 (0.7) | 23 | 7.1 (0.5) | 9.10 (0.4) | 37 | 12.2 (0.8) | 15.30 (1.8) | 62 |
| GATG | 5.0 (0.8) | 12.40 (0.4) | 50 | 11.7 (4.1) | 19.70 (0.3) | 79 | 17.5 (0.6) | 26.10 (1.3) | 105 |
| GACC | 24.6 (4.4) | 7.50 (1.0) | 30 | 14.6 (3.9) | 7.90 (0.6) | 32 | 13.2 (3.6) | 7.30 (2.0) | 29 |
| GACT | 21.0 (1.3) | 1.24 (0.29) | 5 | 61.9 (13.9) | 2.10 (0.6) | 8 | 15.5 (2.4) | 8.50 (1.5) | 34 |
| GACA | 3.2 (0.6) | 0.13 (0.07) | 0.5 | 15.6 (1.7) | 0.11 (0.06) | 0.4 | 10.5 (0.1) | 0.46 (0.09) | 1.9 |
| GACG | 3.2 (0.4) | 0.10 (0.02) | 0.4 | 5.9 (1.9) | 0.14 (0.03) | 0.6 | 10.0 (2.3) | 0.36 (0.04) | 1.5 |
Standard error values are in parentheses.