Table 1.
Effects of a cmt3 mutation on patterns of PAI promoter cytosine methylationa
| Strain
|
PAI gene
|
CG
|
CNG
|
Other C
|
Total C
|
|---|---|---|---|---|---|
| WS | PAI1 | 115 (100%) | 61 (100%) | 149 (100%) | 325 (100%) |
| cmt3b | PAI1 | 76 (66%) | 0 (0%) | 11 (7%) | 87 (27%) |
| WS | PAI2 | 122 (100%) | 53 (100%) | 184 (100%) | 359 (100%) |
| cmt3 | PAI2 | 112 (92%) | 4 (8%) | 45 (25%) | 161 (45%) |
a
The numbers of methylated cytosines in the indicated sequence contexts for eight independent top strand bisulfite sequencing clones for the indicated PAI promoter regions of the indicated strains are shown. The wild-type Wassilewskija (WS) data are derived from data published previously in Luff et al. (1999). The cmt3 data are from this work, and are shown in diagram form in Figure 3.
b
The sequenced DNA was from the WS pai1C251Y cmt3G456D strain.