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Specific phosphorylation of E2F1 by ATM/ATR. (A) Wild-type ATM or catalytically inactive ATM (ATMki) was immunoprecipitated from transfected cells 293T cells and used to phosphorylate GST–p53 (aa 1–70), or GST–E2F1, GST–E2F2, and GST–E2F3 proteins in in vitro reactions. The upper panel depicts the 32P autoradiograph and the lower panel shows an immunoblot with GST antibody. (B) Similar assays as described in A were performed with wild-type ATR or catalytically inactive ATR (ATRki).
