Figure 5.

S6K1 action on gluconeogenesis is dependent on PGC-1α. Primary hepatocytes were treated with vehicle, 10 μM forskolin (Fsk) for 4 h, 20 nM rapamycin for 8 h, or Fsk/rapamycin before RNA and protein were extracted. (A) RNA was subjected to qRT–PCR analysis for the indicated genes. (B) Endogenous PGC-1α was immunoprecipitated from whole-cell extract using anti PGC-1α antibodies. Total proteins and immunoprecipitated material were subjected to Western blot analysis using the indicated antibodies. (C) Primary hepatocytes infected with adenoviral vectors expressing shGFP or shPGC-1α were treated as in A. Total RNA was extracted and subjected to qRT–PCR analysis for the genes indicated. (*) P < 0.05.