Figure 2.

HDACs are enriched at replication forks and deacetylate newly deposited histone H4 regardless of fork movement. (A–E). Cells were labeled with EdU for 10 min followed, by a chase into thymidine-containing medium for the indicated times prior to performing iPOND. (B) Quantitation of H4 acetylation levels compared with total H4 in the click reaction samples from three independent experiments. Error bars in all figures are standard deviations. (C,D) Anacardic acid (30 μM) was added to the indicated samples. (E) HU (3 mM) was added to the indicated samples. (F) Cells labeled with EdU were chased into 3 mM HU medium with or without 100 nM FK228 prior to performing iPOND.