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iPOND monitors post-translational modifications and recruitment of DDR proteins to stalled and collapsed replication forks. (A–D) Cells were labeled with EdU for 15 min (A) or 10 min (B–D), followed by a chase into HU for the indicated times prior to performing iPOND. (D) HU-treated cells were additionally coincubated with or without the Mre11 inhibitor mirin (100 μM) as indicated.
