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γH2AX spreads from a stalled replication fork. (A–D) Cells labeled with EdU for 10 min were chased into thymidine-containing medium prior to addition of HU, then processed using iPOND. The length of the thymidine and HU treatments is indicated. Quantitation of the click reaction samples in C at the 2-h HU-treated samples is from three independent experiments, and at the 1-h HU-treated samples is from two independent experiments.
