Figure 1.

Recruitment of SAGA to the GAL1 upstream activating sequence (UAS). Yeast strains were grown at 30°C in 1% yeast extract containing 2% peptone plus 2% glucose or galactose as indicated. Formaldehyde-based in vivo cross-linking/immunoprecipitation was performed as previously described (Li et al. 2000). Primer-pairs located in the UAS or core promoter of GAL1 and RPS5 (see Materials and Methods) were used for PCR analysis of the immunoprecipitated DNA samples. A PCR fragment corresponding to the GAL4 ORF was used as a control for background binding. Immunoprecipitation was performed using a mouse monoclonal antibody against the c-myc epitope-tag (9E10; Santa Cruz) or a polyclonal antibody against the indicated TAF_II. The promoter, primer-pair, and media are indicated on the left. The ratio of immunoprecipite over the input is indicated below each band. Ratios ≤0.01 are designated .01. IP, immunoprecipitate.