Fig. 2.

Calorie restriction leads to hypermetabolic state. a Oxygen consumption by mitochondria from calorie-restricted cells consume more oxygen. b Mitochondrial H₂O₂ production using succinate (5 mM) as substrate and antimycin (10 μM) and myxothiazol (30 μM) as inhibitors. c External NADH dehydrogenase activity. d Internal NADH dehydrogenase. e Mitochondrial H₂O₂ production using NADH and pyruvate/malate as the substrates.*p < 0.05, **p < 0.01