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. 2001 Aug 1;15(15):1913–1925. doi: 10.1101/gad.903001

Figure 1.

Figure 1

Dedifferentiation of cultured astrocytes to glial progenitors caused by overexpression of PDGF-B chain. (A) The proliferation rate of primary astrocytes (from Gtv-a mouse) was significantly increased by overexpression of PDGF-B. The details of generating growth curve are described in Material and Methods. (B) Giemsa staining shows the morphology of cultured cells infected with RCAS–LacZ and RCAS–PBIG from Gtv-a, Ink4a–Arf+/+ mouse. Fluorescence microscopy shows that the cells infected with RCAS–PBIG are expressing green fluorescent protein. Both photomicrographs are 100× magnification; bars, 100 μm. (C) Western blot analyses show the increased expression of molecular markers for glial precursors in the astrocytes after RCAS–PBIG infection. Two lines of RCAS–lacZ-infected astrocytes were used as control. (PLP) Myelin proteolipid protein; (PDGFR) platelet-derived growth factor receptor; (GFP) green fluorescent protein; (Id4) inhibitor of binding 4, a dominant negative HLH protein. α-Tubulin was used as loading control.