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SDS–PAGE of purified UDGase. Lane 1, positions of molecular mass standards; lane 2, soluble BL21(DE3)/pETTcung cell extracts without IPTG induction; lane 3, soluble BL21(DE3)/ pETTcung extracts after 2 h induction with IPTG; lanes 4 and 5, fractions of purified UDGase after affinity column elution.
