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Assay of T.cruzi UDGase. Closed squares correspond to ethidium bromide fluorescence of cccDNA after heating the samples at 95°C for 8 min. Open squares correspond to ethidium bromide fluorescence after treating the samples with 2 vol DMSO. The data at time 0 were normalized to 100%. (A) Assay using uracil-containing cccDNA. (B) Assay using control cccDNA.
