. 2011 Jul;80(1):97–109. doi: 10.1124/mol.111.071308

TABLE 3.

Abl kinase inhibition with STI571 and broad-spectrum tyrosine kinase inhibition with herbimycin A differentially affect α3^*-nAChR distribution

Analysis of confocal images from CG neurons in 4-day cultures immunolabeled with mAb35 and mAbh10 to detect α3^*-nAChR clusters and their overlap with SV2-containing presynaptic terminals, respectively. nAChR clusters were identified and analyzed in regions of interest (ROIs) from surface (en-face) optical sections as described under Materials and Methods. Overall intensity applies to the mean mAb35 (α3^*-nAChR) fluorescence intensity within selected en-face ROIs; R_m indicates Manders coefficient for α3^*-nAChR/SV2 colocalization. Other parameters indicate α3^*-nAChR cluster or synaptic puncta size, density, and intensity as indicated in the text. STI571 (top) was applied at 10 μM for 24 h; herbimycin A (bottom) was applied at 0.5 μg/ml for 24 h. Both were compared with separate sham-treated controls from the same experiments.

	α3^*-nAChR Labeling				α3^*-nAChR/SV2 Overlap
	Cluster Size	Cluster Density	Cluster Intensity	Overall Intensity	R_m	Puncta Size	Puncta Density	Puncta Intensity
	μm²	μm⁻²	ΔF/F_b	ΔF/F_b		μm²	μm⁻²	ΔF/F_b
Control (n = 11)	0.24 ± 0.03	0.35 ± 0.02	14.5 ± 0.7	2.6 ± 0.5	0.60 ± 0.04	0.12 ± 0.01	0.17 ± 0.02	18.3 ± 0.7
STI571 (n = 10)	0.29 ± 0.03	0.29^* ± 0.02	13.5 ± 0.5	2.2 ± 0.3	0.55 ± 0.05	0.15 ± 0.02	0.16 ± 0.02	17.1 ± 0.5
p Values	0.25	< 0.05	0.26	0.79	0.45	0.20	0.73	0.18
Control (n = 7)	0.22 ± 0.02	0.34 ± 0.01	19.7 ± 1.5	3.5 ± 0.3	N.D.	N.D.	N.D.	N.D.
Herbimycin A (n = 9)	0.14 ± 0.04	0.05 ± 0.01^*	16.1 ± 1.2	0.7^* ± 0.1	N.D.	N.D.	N.D.	N.D.
p Values	0.10	< 0.0001	0.08	< 0.0001

N.D., not done.

Parameter values in the STI571-treated group that are significantly different from those in sham-treated controls (P < 0.05, Student's unpaired two-tailed t test).