Fig. 2.
4-CMTB is a partial agonist at ERK1/2 and is selective for FFA2 compared with FFA3 and FFA1. A, Flp-In TREx 293 cells stably expressing FFA2-eYFP were stimulated for 10 min with varying concentrations of propionate (C3) or 4-CMTB and processed for ERK1/2 activation using the AlphaScreen SureFire ERK1/2 assay (PerkinElmer Life and Analytical Sciences). *, p < 0.05 for 4-CMTB Emax being less than that for propionate according to Student's t test. Data are mean ± S.E.M (n = 3). B, equivalent experiments were performed using hFFA3-eYFP cells (n = 3), where only propionate was an agonist in the ERK1/2 pathway. C, Western blots of hFFA3-eYFP cell lysates treated as for B. Fetal bovine serum [10% (v/v)] was included as a positive control for ERK1/2 activation. D, Western blot as described for C, except that cells expressing FFA1-eYFP were stimulated with 100 μM lauric acid. PT, pretreatment.