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Antagonism of CB1 receptor selectively blocks neurite outgrowth induced by DGL-α expression. The stable DGL-expressing Neuro-2a cells α18 and β14, along with the control P7 cells, were treated for 24 h with 1 μM rimonabant (Rim), a CB1 receptor antagonist, in serum-free media. Cells were then fixed, immunostained using anti-β-tubulin, and subjected to neurite outgrowth assays. ***, P < 0.001 by two-tailed t test.
