Fig. 1.

[³H]Pentamidine uptake in procyclic s427 trypanosomes at a permeant concentration of 30 nM (A) and 1 μM (B). Cells were incubated with [³H]pentamidine for 60 s in the presence or absence of various concentrations of unlabeled pentamidine as indicated. The insets depict the conversion of the inhibition plots to Michaelis-Menten saturation plots. Both graphs are representative of at least six independent experiments, each performed in triplicate. The data shown represent the average of these triplicate determinations ± S.E.M. When not shown, error bars fall within the symbols.