Fig. 3.

Transport of 1 μM [³H]diminazene in procyclic trypanosomes. A, procyclic s427 were incubated for the indicated times with radiolabel in the presence (□) or absence (■) of 1 mM unlabeled diminazene aceturate. The correlation coefficient for the 1 μM line was 0.97 and it was highly significantly different from 0 (P < 0.00001; F test), whereas the 1 mM line was not significantly different from 0 (P = 0.50; F test). B, inhibition of [³H]diminazene transport by various concentrations of pentamidine (●), unlabeled diminazene (○), adenine (▴), and inosine (□). C, Michaelis-Menten saturation curve for [³H]diminazene in procyclic cells, representative of four independent experiments. All experiments were performed in triplicate, and error bars indicate S.E.M.