Fig. 6.

Effect of T0070907 on 15d-PGJ₂-G-mediated IL-2 secretion by activated Jurkat cells. Jurkat cells (5 × 10⁵ cells/ml) were treated with vehicle (VEH of antagonist, 0.02% DMSO) or T0070907 (0.1 and 1 μM) for 45 min, followed by the addition of CGZ (10 or 20 μM), 15d-PGJ₂-G (2.5 or 5 μM), or vehicle (VEH of agonist, 0.1% EtOH) for 30 min. Cells were then stimulated with 40 nM PMA/0.5 μM ionomycin (P/I) for 24 h. The supernatants were harvested, and IL-2 production was measured by ELISA. The results are the mean ± S.E. of triplicate cultures. Statistical significance is indicated by *, p < 0.05 compared with (VEH of agonist + VEH of antagonist) and †, p < 0.05 compared with VEH of T0070907 within each treatment group. The results are representative of three separate experiments.