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Northern analysis of PHR1 gene expression. A. thaliana plants were grown in complete medium for 5 d, then transferred to medium containing Pi (+P) for 7 d or lacking Pi (−P) for 2 or 7 d. Poly A+-enriched RNA was isolated from these samples and RNA gel blots containing 0.5 μg of these samples were hybridized to the PHR1 probe and subsequently rehybridized to a probe corresponding to the RBP4 gene used as loading control.
