Fig. 2.

Primer coverage pattern against 204 reference dioxygenase genes. Each box indicates a perfect match between the gene and the primer. Primers can be classified into the six classes noted on the left, based on their coverages: class A targets all five reference subclades; class B targets PAH-GN; class C targets PAH-GN and T/B; class D targets T/B; class E targets dioxin dioxygenases in OT-I; class F targets PAH-GP. Figure S1 in the supplemental material expands this figure to show each primer name, its computed degeneracies, and the number of perfect matches to the corresponding gene's nucleic acid and protein identification number.