Table 2.
Bacterial and archaeal diversity in incubated mine timber samples amended with [13C]acetate and H2-13CO2a
| Labeled substrate and closest cultured relative | Similarity (%) | Band letter in Fig. 3 | GenBank accession no. |
|---|---|---|---|
| [13C]acetate | |||
| Bacteria | |||
| Acholeplasma sp. | 95 | A | FR838980 |
| Uncultured Bacteroidetes | 99 | B | FR838984 |
| Clostridium sp. | 99 | C | FR838974 |
| Burkholderia cepacia | 100 | D | FR838994 |
| Hyphomicrobium sp. | 99 | E | FR838953 |
| Hydrogenophaga sp. | 94 | F | FR838955 |
| Desulfovibrio sp. | 95 | G | FR838961 |
| Desulfovibrio africanus | 100 | H | FR838982 |
| Halochromatium sp. | 92 | I | FR838989 |
| Desulfuromonas acetexigens | 95 | J | FR838965 |
| Uncultured Geobacteraceae | 96 | K | FR838964 |
| Archaea | |||
| Uncultured Crenarchaeota | 98 | a | FR838968 |
| Uncultured Crenarchaeota | 98 | b | FR838969 |
| Methanosaeta sp. | 98 | c | FR838959 |
| Methanosarcina barkeri | 99 | d | FR838995 |
| Methanosarcina barkeri | 93 | e | FR838996 |
| H2-13CO2 | |||
| Bacteria | |||
| Acholeplasma sp. | 95 | A | FR838990 |
| Uncultured Bacteroidetes | 99 | B | FR838988 |
| Clostridium sp. | 99 | C | FR838975 |
| Burkholderia cepacia | 99 | D | FR838987 |
| Hyphomicrobium sp. | 99 | E | FR838956 |
| Hydrogenophaga sp. | 94 | F | FR838985 |
| Desulfovibrio sp. | 95 | G | FR838973 |
| Desulfovibrio africanus | 95 | H | FR838999 |
| Archaea | |||
| Uncultured Crenarchaeota | 98 | a | FR838967 |
| Methanosarcina sp. | 100 | b | FR838977 |
| Methanosarcina sp. | 100 | c | FR838978 |
| Methanosarcina barkeri | 99 | d | FR838993 |
a
Detected by denaturing gradient gel electrophoresis of density-resolved DNA gradient fractions. The phylogenetic affiliations of the 16S rRNA genes from microbes that incorporated 13C-labeled substrates are shaded.