Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Characteristicsa | Supplier/reference/accession no. |
|---|---|---|
| Strains | ||
| E. coli O157:H7 | ||
| RM6011 | Human isolate (WI) associated with the 2006 U.S. spinach outbreak | T. Monson |
| RM6049 | Human isolate (CA) associated with the 2006 U.S. spinach outbreak | M. Janda |
| RM6069 | Human isolate (PA) associated with the 2006 U.S. spinach outbreak | W. Chmielecki |
| RM6654 | Human isolate (NM) associated with the 2006 U.S. spinach outbreak | E. Hÿttia-Trees |
| RM6067 | Bagged baby spinach isolate (PA) associated with the 2006 U.S. spinach outbreak | E. Hÿttia-Trees |
| RM9994 | Bagged baby spinach isolate (UT) associated with the 2006 U.S. spinach outbreak | E. Hÿttia-Trees |
| RM9998 | Bagged baby spinach isolate (NV) associated with the 2006 U.S. spinach outbreak | E. Hÿttia-Trees |
| RM10000 | Bagged baby spinach isolate (AZ) associated with the 2006 U.S. spinach outbreak | E. Hÿttia-Trees |
| RM6535 | Human isolate (MN) associated with a 2006 U.S. iceberg lettuce outbreak | B. Juni |
| RM6416 | Water isolate (CA) associated with a 2006 U.S. iceberg lettuce outbreak | R. Mandrell |
| RM6607 | Human isolate associated with the 1993 hamburger outbreak | 44 |
| RM6608 | Ground beef isolate associated with the 1993 hamburger outbreak | 44 |
| MQC108 | In-frame deletion of csgA in strain RM6067 C+ variant (Kmr) | This study |
| MQC111 | In-frame deletion of csgA in strain RM6067 C− variant (Kmr) | This study |
| MQC114 | In-frame deletion of csgA in strain RM6535 C+ variant (Kmr) | This study |
| MQC116 | In-frame deletion of csgA in strain RM6535 C− variant (Kmr) | This study |
| MQC118 | In-frame deletion of csgA in strain RM6607 C+ variant (Kmr) | This study |
| MQC120 | In-frame deletion of csgA in strain RM6607 C− variant (Kmr) | This study |
| E. coli non-O157 | ||
| MC4100 | Parental strain of LSR1, a derivative of E. coli K-12 | 6 |
| LSR1 | MC4100csgG::Tn105 (Cmr) | 32 |
| Plasmids | ||
| pKD46 | Red recombinase plasmid (Ampr) | AY048746 (9) |
| pKD4 | Template plasmid (Kmr) | AY048743 (9) |
a
Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; Ampr, ampicillin resistance. Two-letter U.S. state designations shown in parentheses indicate sources of spinach and lettuce outbreak strains.