Table 1.
Primers used for site-directed mutagenesis
| Primer | Sequence (5′–3′)a | Introduced CYP51 alteration |
|---|---|---|
| Mg51-L50Smut | P-CTTCTCTTCCGTGGCAAGTCGTCCGATCCGCCACTCG | L50S |
| Mg51-D134Gmut | P-CACTCCTGTCTTTGGCAAGGGTGTGGCTTATGATTGTCCC | D134G |
| Mg51-V136Amut | P-CTGTCTTTGGCAAGGATGTGGCTTATGATTGTCCCAATTCG | V136A |
| Mg51-Y137Fmut | P-GCTGACCACTCCTGTCTTTGGCAAGGATGTGGTTTTTGATTGTCCC | Y137F |
| Mg51-Y461Smut | P-CCGAGGAGAAAGAAGACTATGGCTCCGGCCTGGTAAGCA | Y461S |
| Mg51-S524Tmut | P-CAGCAGTTTGTTCACCCGGCCGCTGTCGCC | S524T |
a
P, the 5′ phosphate. Underlining indicates altered nucleotides.