. 2011 Jun;77(11):3830–3837. doi: 10.1128/AEM.00027-11

Table 2.

M. graminicola isolate azole sensitivities

Isolate^b	Location and yr of isolation	MgCYP51 substitutions(s)	Mean EC₅₀ (mg liter⁻¹)^a
Isolate^b	Location and yr of isolation	MgCYP51 substitutions(s)	Epoxiconazole	Tebuconazole	Triadimenol	Prochloraz	Prothioconazole	Propiconazole
IPO323	North Brabant, Netherlands; 1981	None (wt)	0.0019	0.053	0.927	0.027	0.051	0.008
MM20	Aragon, Spain; 2006	None (wt)	0.0017	0.063	0.714	0.016	0.194	0.014
MBC/ST1	UK; 1973	None (wt)	0.0069	0.146	1.45	0.021	0.091	0.022
Bd17	Aragon, Spain; 2006	None (wt)	0.001	0.026	0.366	0.002	0.056	0.005
Mean			0.0028	0.072	0.864	0.016	0.098	0.012
Avg deviation			0.002	0.037	0.324	0.007	0.048	0.0057
Mean RF			1	1	1	1	1	1
Rd9	Reading, UK; 1993	Y137F	0.0263	0.299	18	0.115	0.081	0.141
Rd13	Reading, UK; 1993	Y137F	0.0098	0.212	10.6	0.078	0.046	0.048
Bd13	Aragon, Spain; 2006	Y137F	0.0076	0.176	8.2	0.033	0.01	0.028
4414	Belarus; 2007	Y137F	0.0153	0.36	22.1	0.109	0.092	0.108
Mean			0.0148	0.262	14.73	0.084	0.073	0.081
Avg deviation			0.006	0.068	5.32	0.028	0.018	0.043
Mean RF			5	4	17	5	1	7
CTRL-01	Harpenden, UK; 2001	Y137F, S524T	0.093	0.736	>35	0.626	0.063	0.401
4362	Denmark; 2007	Y137F, S524T	0.145	1.01	26.2	0.451	0.293	0.493
R03-25	Harpenden, UK; 2003	Y137F, S524T	0.205	1.47	30.3	0.682	0.322	0.917
R03-31	Harpenden, UK; 2003	Y137F, S524T	0.058	0.379	23.8	0.433	0.284	0.235
R03-45	Harpenden, UK; 2003	Y137F, S524T	0.067	0.842	14.2	0.491	0.238	0.251
Mean			0.114	0.887	27.9	0.537	0.241	0.459
Avg deviation			0.049	0.282	4.7	0.095	0.072	0.196
Mean RF			41	12	32	34	3	38
Bd12	Aragon, Spain; 2006	L50S, Y461S	0.0404	1.47	13.5	0.134	0.314	0.318
IC6	Ireland; 2003	L50S, Y461S	0.0814	1.28	6.7	0.118	0.553	0.196
R03-6	Harpenden, UK; 2003	L50S, Y461S	0.0401	1.68	13.4	0.212	0.556	0.272
Mean			0.0539	1.48	11.19	0.154	0.474	0.262
Avg deviation			0.018	0.135	3	0.038	0.11	0.044
Mean RF			19	21	13	10	1	22
SAC56.1	Edinburgh, UK; 2006	L50S, V136A, Y461S	0.291	0.198	5.59	0.771	2.28	1.32
RF			104	3	7	48	23	110
IC1	Ireland; 2008	L50S, V136A, Y461S, S524T	0.268	0.372	4.86	1.37	12.8	2.13
IC4	Ireland; 2008	L50S, V136A, Y461S, S524T	0.493	0.493	6.76	0.835	3.88	2.48
IC5	Ireland; 2008	L50S, V136A, Y461S, S524T	0.381	0.134	3.08	0.769	2.29	1.49
I43-2	Carlow, Ireland; 2009	L50S, V136A, Y461S, S524T	1.33	0.151	14.6	0.603	5.66	1.58
Mean			0.618	0.288	7.33	0.894	6.16	1.92
Avg deviation			0.28	0.12	2.91	0.19	2.66	0.31
Mean RF			220	4	8	56	63	160
TAG71-3	Andover, UK; 2009	L50S, D134G, V136A, Y461S, S524T	0.6	0.47	15.8	2.67	>35	1.87
RF			215	7	18	167	>357	156

EC₅₀s are the means of two independent experiments. Mean resistance factors (RF) of isolates carrying the same MgCYP51 variant calculated as the fold changes in mean EC₅₀ compared to mean EC₅₀s of wild-type isolates.

Isolates Rd9 and Rd13 were supplied by Mike Shaw (University of Reading), isolates 4414 and 4362 were supplied by Gert Stammler (BASF), and isolates IC1, IC4, IC5, and IC6 were supplied by Eugene O'Sullivan (Teagase).