Table 3.

Azole sensitivities of S. cerevisiae YUG37:erg11 transformants^a

Construct	Amino acid substitution(s)	Epoxiconazole		Tebuconazole		Triadimenol		Prochloraz		Prothioconazole		Propiconazole		Cycloheximide
		EC50	RF	EC50	RF	EC50	RF	EC50	RF	EC50	RF	EC50	RF	EC50	RF
pYES2-Mg51wt	None	0.0019 ± 0.0002	1	0.0055 ± 0.0019	1	0.046 ± 0.0036	1	0.0047 ± 0.001	1	0.041 ± 0.014	1	0.0037 ± 0.0005	1	0.055 ± 0.0049	1
pYES2-Mg51S524T	S524T	0.017 ± 0.0009	9	0.099 ± 0.0034	18	0.684 ± 0.092	15	0.055 ± 0.007	12	1.36 ± 0.75	33	0.036 ± 0.044	10	0.063 ± 0.0045	1
pYES2-Mg51Y137F/S524T	Y137F, S524T	0.025 ± 0.019	13	0.19 ± 0.11	34	18.3 ± 1.23	398	0.306 ± 0.137	65	0.093 ± 0.009	2	0.056 ± 0.006	15	0.058 ± 0.0022	1
pYES2-Mg51L50S/Y461S	L50S, Y461S	0.0121 ± 0.0056	6	0.287 ± 0.015	52	2.06 ± 0.68	44	0.069 ± 0.027	14	0.073 ± 0.002	2	0.043 ± 0.016	12	0.067 ± 0.024	1
pYES2-Mg51L50S/Y461S/S524T	L50S, Y461S, S524T	0.181 ± 0.024	95	3.48 ± 0.27	632	>50 ± NR	>1,080	0.64 ± 0.075	136	1.15 ± 0.29	28	1.37 ± 0.39	370	0.071 ± 0.003	1
pYES2-L50S/D134G/V136A/Y461S	L50S, D134G, V136A, Y461S	0.019 ± 0.012	10	0.016 ± 0.003	3	0.574 ± 0.24	12	0.087 ± 0.004	19	0.148 ± 0.011	4	0.0093 ± 0.002	3	0.063 ± 0.008	1
pYES2-L50S/D134G/V136A/Y461S/S524T	L50S, D134G, V136A, Y461S, S524T	0.38 ± 0.18	200	0.42 ± 0.049	76	>50 ± NR	>1,080	3.92 ± 0.71	834	14.3 ± 1.67	349	4.49 ± 1.09	1,200	0.066 ± 0.007	1

^aEC₅₀ (mg liter⁻¹) are the means of two independent replicates ± standard deviations. Mean resistance factors (RF) of transformants are calculated as the fold changes in EC₅₀ compared to EC₅₀ of transformants expressing wild-type MgCYP51 (pYES2-Mg51wt). NR, no result.