Table 2.

Primers and probe used for quantitative PCR in this study

Primer or probe	Oligonucleotide sequence (5′–3′)	Oligonucleotide positiona (gene amplified)	Reference or source
Un4(d)	GCATATGATGTAGCGAAACAAGCC	589–612 (cry4Aa)	7
		2800–2823 (cry4Ba)
Cry4R	ACCTGGAACATCTGACAACCAATC	454–477 (cry4Aa)	This study
		2935–2958 (cry4Ba)
Cry4Probe	FAM-ACGACACTCGCTCAAATTCAGTACGCT-TAMRA	514–540 (cry4Aa)	This study
		2872–2898 (cry4Ba)

^aStarting from the first base of the sequence of the respective cry gene in the GenBank database (cry4Aa, accession number AL731825, bases 92986 to 96528, and cry4Ba, accession number AL731825, bases 32597 to 36007).