Fig. 3.

GC-MS of EPA and AA generated in mhΔ5neo^r. mhΔ5neo^r was precultured in a GY medium at 25°C for 2 days, harvested, and washed with 1.75% Sealife. The cells were further cultured at 25°C for 1 day in PD medium supplemented with 0.1 mM ETA or DGLA. Total fatty acids were extracted, and FAMEs were analyzed by GC-MS. (a) FAMEs from mhΔ5neo^r corresponding to the EPA methyl ester on GC. (b) EPA methyl ester standard. (c) FAMEs from mhΔ5neo^r corresponding to the AA methyl ester on GC. (d) AA methyl ester standard.