Table 2.
Fatty acid compositions in mhneor and mhΔ5neor,a
| Fatty acid | Proportion (%) in culture of: |
|||||
|---|---|---|---|---|---|---|
| mhneor | mhΔ5neor | mhneor + ETA | mhΔ5neor + ETA | mhneor + DGLA | mhΔ5neor + DGLA | |
| C14:0 | 2.23 ± 0.05 | 2.32 ± 0.03 | 2.26 ± 0.10 | 2.43 ± 0.07 | 2.22 ± 0.06 | 2.28 ± 0.16 |
| C15:0 | 2.43 ± 0.62 | 2.97 ± 0.96 | 2.48 ± 0.64 | 3.04 ± 0.91 | 2.53 ± 0.63 | 2.96 ± 0.79 |
| C16:0 | 55.2 ± 1.83 | 52.1 ± 3.15 | 54.6 ± 1.56 | 51.8 ± 3.56 | 53.5 ± 2.36 | 52.0 ± 3.41 |
| C17:0 | 0.97 ± 0.22 | 1.19 ± 0.42 | 0.96 ± 0.23 | 1.17 ± 0.41 | 0.99 ± 0.21 | 1.19 ± 0.41 |
| C18:0 | 1.54 ± 0.03 | 1.39 ± 0.13 | 1.56 ± 0.02 | 1.4 ± 0.13 | 1.56 ± 0.03 | 1.42 ± 0.13 |
| DGLA | — | — | — | — | 3.92 ± 0.21 | 1.09 ± 0.7 |
| AA | 0.18 ± 0.04 | 0.21 ± 0.02 | 0.15 ± 0.02 | 0.22 ± 0.02 | 0.14 ± 0.01 | 1.85 ± 0.24 |
| ETA | 0.32 ± 0.02 | 0.04 ± 0.04 | 3.27 ± 0.44 | 0.94 ± 0.5 | 0.39 ± 0.04 | 0.08 ± 0.05 |
| EPA | 0.65 ± 0.04 | 0.94 ± 0.13 | 0.62 ± 0.03 | 2.85 ± 0.35 | 0.60 ± 0.04 | 1.15 ± 0.29 |
| DPA | 5.17 ± 0.05 | 5.61 ± 1.00 | 4.92 ± 0.06 | 5.35 ± 0.97 | 4.92 ± 0.11 | 5.44 ± 0.89 |
| DHA | 31.3 ± 0.93 | 33.2 ± 2.44 | 29.2 ± 0.53 | 30.8 ± 2.52 | 29.3 ± 1.32 | 30.5 ± 1.94 |
a
mhneor and mhΔ5neor cells were cultured at 25°C for 1 day in PD medium supplemented with 0.1 mM ETA, 0.1 mM DGLA, or neither. Total fatty acids were extracted, and FAMEs were analyzed by GC. The values, means of triplicate determinations with SD, represent percentages. —, <0.03%.