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Expression of egfp driven by PGAP in P. pastoris. (A) Reporter plasmid pGHg. (B) The yEGFP expression level was measured by using a microplate reader. Reporter strain G/GHg (gray) and control strain G/GH (white) transformed with the empty vector pGH were grown in BMD medium in shake flasks for 60 h. The values represent the averages of three independent cultivations. (C) yEGFP expression level illustrated by flow cytometry histograms.
