. 2011 Jun;77(11):3600–3608. doi: 10.1128/AEM.02843-10

Table 1.

Plasmids used in this study

Plasmid	Description	Source and/or reference
pPICZAgfp	PCR product yEGFP cut with NotI/XhoI was cloned into the NotI/SalI sites of pPICZ A; Zeo^r; 4.0 kb	This study
pGAPgfp	Derivative of pPICZAgfp in which the BglII/NotI P_AOX region was replaced by the PCR product P_GAP; Zeo^r; 3.5 kb	This study
pGHg	Derivative of pGAPgfp in which the BamHI/BglII fragment from pAO815 was inserted into the BglII site; Zeo^r; 7.6 kb	This study
pGH	Derivative of pGHg in which the egfp gene was removed by HindIII digestion; Zeo^r; 6.8 kb	This study
pSAOH5	Expression vector containing the E. coli lacZ gene coding for β-galactosidase; Amp^r; 10 kb	39
pDS56	Vector containing the methionine adenosyltransferase (MAT) gene (ds56) obtained through DNA shuffling; Amp^r; 3.5 kb	17