Table 3.

Impact of promoter strength on the expression of different genes^a

Promoter	ds56			egfp		lacZ
	Transcript level	MAT activity (U/mg of protein)	Volumetric production of SAM (g/liter)	Transcript level	yEGFP fluorescence (RFU/OD600)	Transcript level	Gal activity (U/mg of protein)
G7	0.09 ± 0.04	0.10 ± 0.02	0.04 ± 0.01	0.05 ± 0.06	0.17 ± 0.08	0.16 ± 0.08	0.26 ± 0.05
G6	0.50 ± 0.17	0.34 ± 0.03	0.24 ± 0.05	0.14 ± 0.12	0.49 ± 0.11	0.71 ± 0.21	0.5 ± 0.07
GAP	1.00 ± 0.24	1.00 ± 0.13	1.00 ± 0.11	1.00 ± 0.16	1.00 ± 0.13	1.00 ± 0.22	1.00 ± 0.14
G5	1.89 ± 0.29	1.31 ± 0.15	1.34 ± 0.38	2.22 ± 0.42	1.25 ± 0.15	2.17 ± 0.53	1.35 ± 0.25
G4	6.72 ± 1.31	2.29 ± 0.28	5.33 ± 0.52	8.43 ± 1.22	4.29 ± 0.64	9.72 ± 1.74	5.48 ± 0.64
G3	15.52 ± 2.12	3.99 ± 0.64	8.63 ± 0.78	16.74 ± 2.08	7.75 ± 0.57	22.52 ± 3.76	18.95 ± 2.74
G2	39.18 ± 5.18	4.72 ± 0.36	11.04 ± 0.40	35.64 ± 4.24	12.14 ± 1.24	42.57 ± 6.76	51.38 ± 4.85
G1	64.04 ± 7.47	5.70 ± 0.34	10.56 ± 0.21	47.78 ± 5.72	19.58 ± 1.43	74.14 ± 8.52	96.16 ± 7.78

^aRelative yEGFP fluorescence, Gal specific activities, MAT specific activities, volumetric production of SAM, and transcript levels of reporters were normalized to the value obtained with the wild-type P_GAP. Wild-type values were set to 1.