Fig. 5.

ACCM-2 supports culture of C. burnetii from infected animal tissue. ACCM-2 was inoculated with a 1:100 dilution of total lung, liver, or spleen homogenates derived from NMII-infected SCID mice infected by intraperitoneal or intratracheal routes as described in Materials and Methods. Closed symbols indicate the starting numbers of GE/ml. Open symbols indicate the numbers of GE/ml after a 7-day incubation. Depicted data are the means of three ACCM-2 cultures individually started with tissue homogenates from three infected mice.