Fig. 2.

Cell division and biofilm formation. PS1, PS2, and PS3 were grown in beef broth on 96-well microtiter plates at 10°C for 10 days. The growth medium was carefully aspirated; serial dilutions were plated on SMAC-strep agar plates to obtain cell counts. The cell counts were indicative of cell division. The results are reported as CFU/ml (A). Biofilms were washed twice with PBS and quantified with the ATP assay. Biofilm amounts are given as RLU (B). The experiment was done with five replicates from each of three independently grown bacterial cultures.