Table 1.
Strains and plasmids used in this study
| E. coli strain or plasmid | Relevant genotypea | Source or reference |
|---|---|---|
| Strains | ||
| ATCC 43894 | E. coli O157:H7 | American Type Culture Collection (14) |
| O157:H− | flhC | 12 |
| PS1 | E. coli O157:H7 Strr Nalr | This study |
| PS2 | E. coli O157:H− Strr | This study |
| PS3 | PS2 pXL26 | This study |
| Plasmid | ||
| pXL26 | pT7-7 flhC | 33 |
a
The designation “H−” refers to the lack of the H7 flagellin due to the flhC mutation, which renders the entire flagellar master regulator complex, FlhD/FlhC, dysfunctional.