Table 3.

Microarray analysis of PS1 and PS2 grown on the surface of beef

ORF (total no. of genes)a	Geneb	Protein functionc	Expression ratiod	z-test P valuee
Metabolism (125)
b3236	mdh*	Malate dehydrogenase	0.15	5.5 × 10−10
ECS0753	sucC	Succinate dehydrogenase	0.17	2.6 × 10−5
Z0877	sdhA*	Succinate dehydrogenase	0.28	7.7 × 10−4
b0430	cyoA*	Cytochrome o-oxidase	0.25	0.0032
b0432	cyoC	Cytochrome o-oxidase	0.14	1.27 × 10−4
Transport (34)
Z1276	ompF*	Outer membrane protein F	0.47	0.0098
Z3473	ompC*	Outer membrane protein C	0.13	2.74 × 10−5
ECS1744	oppB*	Oligopeptide permease	0.38	0.0009
Z3570	hisQ	Histidine permease	0.39	0.0187
ECS5330	osmY	Hyperosmotically inducible periplasmic protein	0.20	6.1 × 10−4
b4123	dcuB	Anaerobic dicarboxylate transporter	0.29	0.0548
Cell division (7)
b0039	ftsW	Membrane protein involved in shape determination	0.085	1.93 × 10−9
ECS0975	ftsK*	Cell division	0.14	8.06 × 10−9
Z0105	ftsZ*	Tubulin-like GTP binding protein	0.43	0.0032
Z4333	ftsY*	Cell division membrane protein	0.33	1.6 × 10−4
b0924	mukB	Kinesin-like cell division protein	0.093	7.7 × 10−7
Z5336	minC*	Inhibits ftsZ ring formation	0.30	0.057
ECS1670	chpB	Probable growth inhibitor	0.40	0.0062
LEE pathogenicity island (10)
Z5119	escN*	Type III secretion system	0.35	0.0047
Z5112	tir*	Translocated intimin receptor	0.10	4.03 × 10−9
Biofilm (2)
b0196	rcsF*	Regulator in colanic acid synthesis	0.38	0.0025
b2058	wcaB*	Putative transferase	0.44	0.0087
Miscellaneous
b0438	clpX*	ATP-dependent specificity component of ClpX/ClpP complex	0.23	6.17 × 10−5

^aOpen reading frames (ORF) are taken from E. coli K-12 MG1655 (7), E. coli O157:H7 EDL933 (36), and E. coli O157:H7 Sakai (20).

^bAsterisks indicate genes that were selected for real-time PCR.

^cProtein functions were determined with the Kyoto Encyclopedia of Genes and Genomes (KEGG; http://www.genome.jp/kegg).

^dThe expression ratio (or fold difference) was determined by dividing the pixel values of the parent strain by those of the flhC mutant for each experiment. The average over five experiments is presented.

^ez-tests were performed on the expression ratios obtained, and ratios having a P value of ≤0.05 were selected.